Chromatographic Analysis of Olopatadine in Hydrophilic Interaction Liquid Chromatography
نویسندگان
چکیده
منابع مشابه
Chromatographic analysis of olopatadine in hydrophilic interaction liquid chromatography.
In this paper, chromatographic analysis of active substance olopatadine hydrochloride, which is used in eye drops as antihistaminic agent, and its impurity E isomer by hydrophilic interaction liquid chromatography (HILIC) and application of design of experiments (DoE) methodology are presented. In addition, benzalkonium chloride is very often used as a preservative in eye drops. Therefore, the ...
متن کاملHydrophilic interaction liquid chromatography (HILIC) in proteomics
In proteomics, nanoflow multidimensional chromatography is now the gold standard for the separation of complex mixtures of peptides as generated by in-solution digestion of whole-cell lysates. Ideally, the different stationary phases used in multidimensional chromatography should provide orthogonal separation characteristics. For this reason, the combination of strong cation exchange chromatogr...
متن کاملCharacterization of intact neo-glycoproteins by hydrophilic interaction liquid chromatography.
In this study, an HPLC HILIC-UV method was developed for the analysis of intact neo-glycoproteins. During method development the experimental conditions evaluated involved different HILIC columns (TSKgel Amide-80 and ZIC-pHILIC), and water-acetonitrile mixtures containing various types of acids and salts. The final selected method was based on a TSKgel Amide-80 column and a mobile phase compose...
متن کاملHydrophilic interaction chromatography
In the early 1990s, hydrophilic interaction (liquid) chromatography (HILIC) emerged as a new separation method. HILIC is becoming increasingly popular for the separation of polar compounds in aqueous-organic mobile phases rich in organic solvents (usually acetonitrile). It has become important for the separation of pharmaceuticals, neurotransmitters, nucleosides, nucleotides and other compounds...
متن کاملAn arginine-functionalized stationary phase for hydrophilic interaction liquid chromatography.
An arginine-functionalized stationary phase for hydrophilic interaction liquid chromatography (HILIC) has been prepared for the first time by clicking arginine onto silica gel. It offers an efficient separation of organic acids, nucleotides and sugars. More interestingly, it exhibited excellent selectivity and enrichment toward acidic glycopeptides, even at a ratio of 1 : 150 to non-glycopeptides.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Chromatographic Science
سال: 2014
ISSN: 1945-239X,0021-9665
DOI: 10.1093/chromsci/bmu102